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RainDance Technologies RDT 1000
RDT 1000 increases efficiency of target sequencing #3
RDT 1000 increases efficiency of target sequencing #2
RDT 1000 increases efficiency of target sequencing #1
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Primer library production process
Droplet-based PCR process – PCR reaction mix containing fractionated genomic DNA is loaded into the RDT 1000 sequence enrichment chip along with the PCR primer library. The instrument generates droplets containing a gDNA + PCR mix and pairs them with the primer droplets. The paired droplets flow past an electrode embedded in the chip and are instantly merged together. The resulting PCR droplets are then automatically dispensed into a PCR tube which is transferred by the user to a standard thermal cycler for PCR amplification (A).
The uniformity and stability of the droplets are shown in a micrograph of the droplet emulsion (B). Amplification products are purified by breaking the emulsion and purified using a PCR clean-up kit. An aliquot of the purified products was analyzed by capillary electrophoresis to quantify product yield. The resulting electropherogram is compared to a calculated peak profile based on the predicted size and number of amplicon products in the PCR library (C). The comparison is used as a QC step prior to DNA sequencing (D).
RDT Workflow Diagram
S. Roopom Banerjee, President and Chief Executive Officer
Christopher McNary, Chief Commercial Officer
Darren R. Link, Ph.D., Co-Founder and Vice President, Research and Development
Patrick Kelly, European Sales Director
Jeff Olson, Manager of Nucleic Acid Applications
