FAQ – Sample Preparation for Sequencing
- How are the PCR products sequenced using short-read sequencing platforms?
- How are the PCR products sequenced using long-read sequencing platforms?
Q – How are the PCR products sequenced using short-read sequencing platforms?
A – The current procedure recommended by RainDance involves ligating amplicons to form high molecular weight concatamers followed by random fragmentation using physical shearing of the DNA to generate fragments suitable for library preparation.
Q – How are the PCR products sequenced using long-read sequencing platforms?
A – RainDance recommends following the amplicon sequencing protocol provided by the sequencing platform vendor.
